Journal: FEBS Open Bio

Article Title: Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease

doi: 10.1002/2211-5463.13021

Figure Lengend Snippet: (A) Cells were isolated from murine hearts, stained with antibodies, and put through fluorescence‐activated cell sorting (FACS) and gated with negative markers for CD31/34/45 and positive markers for NG2, PDGFRβ, and CD146. (B) Bright‐field images of cultured cells passaged after isolation showing typical PC morphology. (C) Proliferation curve of primary PCs in DMEM and EGM‐2. There was no significant difference between the two medias used on their proliferation. (D) Protein expression of typical PC markers. Data are presented as the mean ± SD. Scale bar = 1000 µm.

Article Snippet: Pericytes were seeded at 8000 cells/mL into two 6‐well cell culture plate (Corning Inc., Corning, NY, USA) precoated with gelatin (Cell Biologics Inc.) in either Dulbecco's modified Eagle media (DMEM) with 4.5 g·L −1 glucose, l ‐glutamine, and sodium pyruvate (Corning Inc.) supplemented with +5% FBS (Corning Inc.) +1% penicillin/streptomycin (P/S) (Corning Inc.) or endothelial growth medium 2 (EGM‐2; Cell Biologics, Inc., Chicago, IL, USA) supplemented with the ECs medium supplement kit (Catalog M1168, Cell Biologics Inc., Chicago, IL, USA).

Techniques: Isolation, Staining, Fluorescence, FACS, Cell Culture, Expressing